Three different deep-seawater (DSW) treated by means of reverse osmosis (RO DSW), electrodialysis (ED DSW) and 10% (v/v) dilution with ddH2O (10% DSW) were as treated groups, while distilled water (NDW) was the control group. Body weight changes, feed and water intakes of high-cholesterol dietary mice were not (P < 0.05) influenced by drinking different waters. Although drinking ED and 10% DSW lowered (P < 0.05) serum cholesterol, triglyceride (TG) and non-high-density lipoprotein cholesterol (non-HDL-C) levels than NDW, the HDL-C/non-HDL-C ratio was only improved (P < 0.05) in the ED DSW group. These hypolipidemic effects of ED and 10% DSW might result from more (P < 0.05) fecal cholesterol/TG outputs compared with NDW and RO DSW groups. The rich K+ and Mg2+ contents, as well as better Mg2+/Ca2+ ratios in ED and 10% DSWs may counteract increased blood pressures of high-cholesterol dietary mice due to a higher hypertensive mineral, Na+.